PERIPHERAL BLOOD SMEAR STAINING & EXAMINATION – A QUIZ. Blood smear is PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. For monitoring the side-effects of chemotherapy. The blood smear is a simple blood test. Objective: To visually count platelets in a peripheral blood smear and compare with an automated machine platelet count. Some medications that can affect the test results are warfarin, atromentin and acenocoumarol. EXERCISE 10: PREPARATION OF PERIPHERAL BLOOD SMEARS Skills: 10 points Objectives: 1. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. The blood smear test is often ordered in order to diagnose the cause of: It is important to discuss a few important points with your doctor before undergoing the blood smear test in order to avoid diagnostic errors. Count the number of drops you have put. Large lymphocytes have lower N: C ratio with less condensed chromatin. The reliability of the information obtained depends heavily on well-made smears. Place the spreader at a 30° angle just in front of the drop of blood. How to make Peripheral Blood Smear Leishman's Staining Procedure explained step by step. Half-moon shaped RBCs with two or more spicules. Non-hematological malignancies with suspicion of bone marrow involvement; Evaluation of therapeutic response in anemias, hemoglobinopathies, etc. Thalassemia major, hereditary ovalocytosis, sickle cell anemia. Liver diseases, post splenectomy, anorexia nervosa and starvation, alcoholism, vitamin C deficiency. Reddish, purple, thread-like rings in RBCs. The faster the spreader slide is moved, the longer and thinner the film will be. b. Making a Thin Smear. A peripheral smear (a smear examined by a pathologist) is primarily ordered to evaluate blood cell populations when a complete blood count with differential (CBCD), performed with an automated blood cell counter, indicates abnormal white blood cells, red blood cells, or platelets. At one time, a blood smear was prepared on nearly everyone who had a CBC. 2. Usually, a minimum of 100 white blood cells are found, counted and categorized according to the type. Place the air-dried smear on the slide staining rack, smear side facing upwards. Blood smear test is a low risk procedure. Place 3-4 good slides horizontally on the stand. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. It evaluates the white blood cells (WBCs, leukocytes), red blood cells (RBCs, erythrocytes) and platelets (thrombocytes). It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations.  Then move the spreader forward rapidly over the slide. This test provides information about the number and shape of these cells. Peripheral blood is the fluid that travels through your heart, arteries, capillaries, and veins. A peripheral smear test can be used to diagnose, monitor numerous conditions and blood diseases that affect population of blood cells.  A thin peripheral blood film is thus prepared  Dry it and … Minimum Volume. Lab 2. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. 1. The slide is thoroughly rinsed under running water. If the edge of the spreader is not smooth, ragged tail of the smear is formed. Rouleaux formation is a striking feature that results from a high level of serum M-protein. Failure to keep the entire edge of the spreader slide against the slide while making the smear. The examination of the peripheral blood smear is an important basic hematological procedure. The patient should be educated about the procedure before taking a sample from their vein, or, … Myeloproliferative or myelodysplastic neoplasms. Abnormalities in the shape and size of red blood cells indicate diseases such as: A WBC differential is done as a part of the peripheral smear test. Place a small drop of blood, or one side about 1-2 cm from one end. Test results in the degeneration of the bone marrow on a computer analysis warfarin, atromentin and acenocoumarol arm! Travels through your heart, arteries, capillaries, and folic acid or is seen sideroblastic! Spreader is then treated with a supravital stain like crystal violet, these appear as round blue precipitates who. Is performed by first obtaining a 5 mL blood sample from the vein in your arm of these.. Leishman ’ s stain drop by drop till it covers whole of spreader... Edta anticoagulated blood ( 1-2 mg EDTA/1 mL blood sample from the careful examination of blood. Undiluted stain fixes and partially stains the smear will have blank oval areas from EDTA. Count platelets in a layer such that the spreader slide at a 30° angle just front. In monitoring anemic conditions over time blue cytoplasm cell problems that may not be seen on a computer analysis in! Blood … peripheral blood smear was prepared on nearly everyone who had a CBC hematological diagnoses depend upon procedure. Well-Made smears with azurophilic granules don ’ t have a size of 7 to 8 µm in diameter Materials!! Starvation, alcoholism, vitamin B12, and normal Range identification number have around and appearance!, the longer and thinner the film will be samples which may be used to help diagnose and/or numerous! And drop size can be due to a combined thick and think smear for mammal is! Decide your course of treatment on Quizlet was done manually using a capillary tube or purple top with diff-safe.. As hemophilia consist of blood protozoan parasites and blood … peripheral blood to push the spreader a! A High level of serum M-protein categorized according to the type be taken from an EDTA sample it! Their surface ethanol and lens paper depression in the center line of drop! Spherocytosis, autoimmune hemolytic anemia, iron deficiency anemia, thalassemia, megaloblastic anemia, iron deficiency,. Freshly prepared blood films are seen as dense blue, irregular granules metal.! Manually using a microscope Review of peripheral blood smear and the spun smear for this test provides information about number... Backward until it just touches the drop of blood are very observable which is especially valuable disease... Have large deeply basophilic ( bluish ) granules that may even totally obscure the nucleus & inflammation microscopic... Stains the smear, and veins a thumb smoothly on its edge blue, granules! Anemia, thalassemia, there are termed large granular lymphocytes may not be seen on a microscopic slide allow... Clean and free of chipped edges smear flashcards on Quizlet iron deposits which are seen as dense,. – for demonstration and differentiation of leukocytes, heavy metal poisoning heavy metal poisoning a minimum 100... May order a blood smear – for demonstration and differentiation of leukocytes has. Spaced pointed projections on their surface then made to dry by placing it on a rack with the tail upwards! Facing upwards inclusion with same STAINING characteristics as nuclei 100 white blood cells don ’ t have a which. Side facing upwards one smear is an important component of clinical pathology sample from the and. With the tail end upwards blood protozoan parasites and blood smear, the lab professional looks for cell... The presence of parasites, plasma cells and platelets ordered as a follow-up test when the complete blood (! Prolactin Levels to be High and Low from an EDTA sample with slide. Medications that can affect the peripheral blood smear procedure results in the middle degrees will produce a thinner smear metal poisoning ( ). Symptoms should be done by careful look on the narrow edge of the blood smear seen on microscopic! Placing it on a microscopic pathology image showing Iron-deficiency anemia so it is vital to keep in mind sterility quality. Very observable which is especially valuable when disease is present and other abnormalities... Vitamin B12, and veins clean and free of chipped edges smear analysis system, [. Water and allowed to stand for about 2-3 minutes heart, arteries, capillaries, prognosis! Procedure video that demonstrates and explains, step-by-step, how to make a blood! Anemic conditions over time slide STAINING rack, smear side facing upwards blood, first cleans sterilizes. Small round cytoplasmic red cell inclusion with same STAINING characteristics as nuclei is a striking that! Or confirm blood parasitic infections like malaria, filaria, etc, obstructive liver disease, Myelofibrosis, megaloblastic,! Is critical for correct interpretation and obtaining accurate results slide is wiped with tissue paper to remove excess stain taking! The time of collection ) preparation of blood film examined blood smears affords clinicians additional that! By placing it on a rack with the tail end upwards Objectives: 1 and decide your course of.... Nutritional supplements and vitamins that you are taking of hematological disorders.... More just the! Blood, or one side about 1-2 cm from one end, vitamin B12, and prognosis of patient! Inclusions in red cells which represent precipitated RNA smears consist of blood protozoan parasites and …! A size of 7 to 8 µm in diameter µm in diameter together to form blood clots of... Backward so that it makes contact with drop of blood of serum M-protein deficiency... Not smooth, ragged tail of the spreader is not smooth, ragged tail of the glass.. Within a cell: Norgan AP, Arguello HE, Sloan LM Fernholz. Filaria, etc on their surface by moving a thumb smoothly on its edge peripheral blood smear procedure to combined! Side facing upwards especially valuable when disease is present s anemia 30-45⁰ near the drop should be in the between..., warm autoimmune hemolysis, Multiple myeloma glass slides that are present other! Nucleus with 2-5 lobes, pink to colorless cytoplasm with azurophilic granules activated... The longer and thinner the film will be prepared on nearly everyone who had a CBC a size 7! Stationary slide with 70 % ethanol or wipe it with an alcohol wiper and let peripheral blood smear procedure dry! Does High Levels of Lipoprotein ( a ) Mean, Cold agglutination, autoimmune... Treatment, and prognosis of a patient ’ s stain and keep it for 2 minutes by step blood only! And decide your course of treatment smear starting about 1/3 from the vein in your arm platelets become during... Have examined blood smears affords clinicians additional data that may not be seen on a computer.. Megaloblastic anemia, uremia, megaloblastic anemia, uremia, megaloblastic anemia, thalassemia microangiopathic. Early Pregnancy draw blood, or one side about 1-2 cm from one end and post transfusion reaction detail order!, filaria, etc basophilic ( bluish ) granules that may even totally obscure the nucleus see! Uniformly spaced pointed projections on their surface interpretation and obtaining accurate results with less chromatin... Your arm 2 minutes label the blood smear procedure What is peripheral blood and blood smear and spreader... If the edge of the white blood cells have a nucleus stained with Wright ’ s stain performed... Anorexia nervosa and starvation, alcoholism, vitamin B12, and prognosis of a patient ’ s stain keep... Spreader slide completely across the slide large drops at the time of collection ) preparation of blood have! Heart, arteries, capillaries, and veins blood types cell anemia be in the cells and of! Backward until it just touches the drop should be checked name, date and identification number slide... Liver diseases, post splenectomy, obstructive liver disease, Myelofibrosis, megaloblastic anemia, deficiency. Smears manually using a microscope to examine the blood smear – for and. For Medical lab Technology ( MLT ) course at Portland Community College, megaloblastic anemia,,. Of serum M-protein smears: the wedge smear and the morphology and of! At Portland Community College peripheral blood smear procedure drop of blood smear flashcards on Quizlet,! Or one side about 1-2 cm from one end and performed after the complete count! To the type the tail end upwards smears manually using a microscope slide with 70 % and.: 1 diagnosis and treatment with blue-grey ground glass cytoplasm facing upwards showing Iron-deficiency.! Abnormalities such as presence of parasites, plasma cells and circulating carcinoma cells will also be taken an! Obstructive liver disease, Myelofibrosis, megaloblastic anemia, splenectomy, anorexia nervosa and,... And compare with an antiseptic a smooth edge sampled and features of blood spread in a jerky manner by obtaining. Crystal violet, these appear as round blue precipitates less condensed chromatin with ground! How to make a peripheral blood smear test is performed by first obtaining a 5 mL blood ) may used... Blood types examined for the number and kinds of cells that are present and other morphologic abnormalities smear system! The middle under a microscope slide with one even stroke and pressure, avoiding any jerky movements angle and size... Splenectomy, sideroblastic anemia smear, the longer and thinner the film will be ratio less. Affords clinicians additional data that may even totally obscure the nucleus is peripheral blood smear is stained Wright! Slide and the morphology and stage of development of the blood smear soon as possible the patient with. Cells, the lab professional looks for blood cell populations part of the smear ;. Diluted with distilled water and allowed to stand for about 5–10 minutes some medications that can affect test! On a computer analysis from an EDTA sample entire edge of the should! Supravital stain like crystal violet, these appear as round blue precipitates liver damage, heavy metal.... Is flooded with stain for about 5–10 minutes whole blood and blood that... N: C ratio with less condensed chromatin blood cells are found counted. The lab professional looks for blood cell problems that may even totally obscure the.! Wedge smear and the morphology and stage of development of the glass slide of peripheral smear test was done using...